Remarkable increase in luminol electrochemiluminescence by sequential electroreduction and electrooxidation.
نویسندگان
چکیده
Luminol electrochemiluminescence is dramatically increased by about five hundred times by taking full advantage of both electrochemical reduction and electrochemical oxidation using simple linear sweep voltammetry, leading to sensitive detection.
منابع مشابه
Electrochemiluminescence Bioassays with a Water-Soluble Luminol Derivative Can Outperform Fluorescence Assays.
The most efficient and commonly used electrochemiluminescence (ECL) emitters are luminol, [Ru(bpy)3 ]2+ , and derivatives thereof. Luminol stands out due to its low excitation potential, but applications are limited by its insolubility under physiological conditions. The water-soluble m-carboxy luminol was synthesized in 15 % yield and exhibited high solubility under physiological conditions an...
متن کاملUltrasensitive immunoassay based on a pseudobienzyme amplifying system of choline oxidase and luminol-reduced Pt@Au hybrid nanoflowers.
A multi-functional luminol-reduced Pt@Au hybrid flower-like nanocomposite (luminol-Pt@AuNF) which not only acts as an efficient signal probe but also constitutes a pseudobienzyme amplifying system with choline oxidase (ChOx) was firstly synthesized and applied to the construction of a solid-state luminol electrochemiluminescence (ECL) immunosensor for cardiac troponin I (cTnI) detection.
متن کاملHighly enhanced electrochemiluminescence based on synergetic catalysis effect of enzyme and Pd nanoparticles for ultrasensitive immunoassay.
An immunosensor based on the electrochemiluminescence (ECL) of luminol was proposed by coupling enzymatic reaction to in situ generate coreactant with Pd nanoparticles as catalyst for the ECL reaction, which was successfully applied for the ultrasensitive detection of α-1-fetoprotein with a low detection limit of 33 fg mL(-1).
متن کاملDetection of phosphatidylserine in the plasma membrane of single apoptotic cells using electrochemiluminescence
Phosphatidylserine (PS) in the plasma membrane of single apoptotic cells was detected using luminol electrochemiluminescence for the first time. Membrane PS was converted by aqueous phospholipase D and L-amino acid oxidase to generate hydrogen peroxide, inducing electrochemically generated luminescence. The successful observation of PS in the cellular membrane provided a special strategy for th...
متن کاملDesign and biosensing of Mg²⁺-dependent DNAzyme-triggered ratiometric electrochemiluminescence.
A dual-potential ratiometric electrochemiluminescence (ECL) sensing approach based on Mg(2+)-dependent DNAzyme-regulated ECL signals of luminol and CdS quantum dots (QDs) is designed. The system consists of DNAzyme strand functionalized QDs as capture probes and cathode ECL emitters, luminol-reduced gold nanoparticles (Au@luminol) as anode ECL emitters, and a Mg(2+) substrate strand modified wi...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Chemical communications
دوره 50 93 شماره
صفحات -
تاریخ انتشار 2014